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Matricaria urea extract exhibits antinociceptive activity in male rat


The aim of the present study was to explore the probable antinociceptive effects of Hydroethanolic extract of Matricaria urea (Mu) in experimental models of acute pain (Tail flick) and chronic pain (Formalin test).

Materials and methods

Effects of Mu extract were assessed with the antinociceptive effects of sodium salicylate (SS) 200 mg/kg as a positive control. The results are expressed as mean ± SEM and the difference was analyzed by means of ANOVA followed by Tukey test.


Antinociceptive of 200 mg/kg of SS (i.p) had no effect on Tail flick latency, while all doses (250, 500 mg/kg) of extract, increased this latency (at least p < 0.05). SS produced antinociceptive only in the Second phases of formalin test (p < 0. 05), while in the first phase it's not effective. All doses of Mu extract showed a significant effect on both phases of formalin test (at least p < 0.01). The lethal dose (LD50) of extract was about 4000 mg/kg.


In generally Mu extract has effect on the Tail-flick latency and both phases of formalin test; the site of action is probably central and the mechanism of antinociceptive of extract remains to be elucidated


  1. Elisabetsky E, Amodor TA, Albuquerque RR, Nunes DS, Carvalho ACT: Analgesic activity Psychotria colorata (Wild. ex R. and S.) Muell. Arg. alkaloids. J Ethnopharmacol. 1995, 48: 77-83. 10.1016/0378-8741(95)01287-N.

    Article  CAS  PubMed  Google Scholar 

  2. D'Amour FE, Smith DL: A method for determining loss of pain sensation. J Pharmacol Exp Ther. 1941, 72: 74-79.

    Google Scholar 

  3. Dubusisson D, Deniss SG: The formalin test: a quantitative study, of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats. Pain. 1977, 4: 161-174. 10.1016/0304-3959(77)90130-0.

    Article  Google Scholar 

  4. Tjolsen A, Berge O, Hunskaar S, Rosland JH, Hole K: The formalin test: an evaluation method. Pain. 1992, 51: 5-17. 10.1016/0304-3959(92)90003-T.

    Article  CAS  PubMed  Google Scholar 

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Khaksarian, M. Matricaria urea extract exhibits antinociceptive activity in male rat. Ann Gen Psychiatry 5 (Suppl 1), S166 (2006).

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