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  • Meeting abstract
  • Open Access

Evaluating the effect of aquatic extraction of Cannabis sativa seed on spatial memory consolidation in rats

  • 1,
  • 1,
  • 2 and
  • 1
Annals of General Psychiatry20109 (Suppl 1) :S139

  • Published:


  • Aquatic Extraction
  • Brain Function
  • Spatial Memory
  • Memory Process
  • Memory Function


The existence of an endocannabinoid system in the central nervous system that consists of G protein-coupled CB1 cannabinoid receptors and endocannabinoids, including arachidonylethanolamide and 2-arachidonoylglycerol, has gained general acceptance. Recent reports suggest that this system may serve several physiological functions including learing and memory functions

Materials and methods

40 male wistar rats (3-4 month, 320-260 g) were completely divided into 4 experimental groups and control group. Cannabis sativa seed was extracted with Soxhlet apparatus. To test spatial memory, Morris water mazemaze (7 days,4 trails) was used. Experimental groups with 50, 100, 150 -1,210 were injected in the peritoneal (IP) orderly and after one hour of injection spatial memory was done.


The result show that experimental groups (50,100,150 -1 doses), for learning time have significant level deduction in the comparison of controlgroup (p < 0.05), but experimental group with 210 dose has not significant level in the comparison of control group (p < 0.05).


We demonstrate tetrahydrocannabinol can change brain function as learning and memory processes and probably was done with Depolarization-Induced Suppression of excitatory (DSE) mechanism in the CA1 area of Hippocamp that with neurotransmitter regulation cause to neuroplasticity.



We thank Dr Heravi and Mr Kazemi for money support and Azad University of Mashhad for prepare Lab for Test.

Authors’ Affiliations

Department of Biology, Faculty of Science, Islamic Azad University, Mashhad Branch, Mashhad, Iran
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran


  1. Lee Jonathan LC: Memory reconsolidation mediates the strengthening of memories by additional learning. Nature Neuroscience. 2008, 11: 1264-1266. 10.1038/nn.2205.View ArticlePubMedGoogle Scholar
  2. Kawamura Y, Fukaya M, Maejima T, Yoshida T, Miura E, Watanabe M, Ohno-Shosaku T, Kano M: The CB1 Cannabinoid Receptor Is the Major Cannabinoid Receptor at Excitatory Presynaptic Sites in the Hippocampus and Cerebellum. Neuroscience. 2006, 26: 2991-3001. 10.1523/JNEUROSCI.4872-05.2006.View ArticlePubMedGoogle Scholar
  3. Kyari MZ: Extraction and Charachterization of seed oils. Int Agrophysics. 2008, 22: 139-142.Google Scholar
  4. Varvel SA, Litchman AH: Evaluation of CB1 receptor knockout mice in the Morris water maze. Pharmacol Exp Ther. 2002, 301: 915-24. 10.1124/jpet.301.3.915.View ArticleGoogle Scholar


© Ebrahimpour et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.