Skip to content

Advertisement

  • Poster presentation
  • Open Access

Effects of nitric oxide synthase inhibition in the CA1 region of rat hippocampus on spatial learning

  • 1,
  • 1,
  • 1 and
  • 1
Annals of General Psychiatry20087 (Suppl 1) :S118

https://doi.org/10.1186/1744-859X-7-S1-S118

  • Published:

Keywords

  • Nitric Oxide
  • Synaptic Plasticity
  • Spatial Learning
  • Morris Water Maze
  • Escape Latency

Background

Nitric oxide (NO) is thought to be involved in synaptic plasticity contributing to learning and memory in several brain areas including the hippocampus [1]. The hippocampus is believed to have a critical role in the processing of spatial information [2]. But, data on the role of hippocampal NO in spatial or other types of learning are not consistent [3, 4]. In the present study the effect of NO synthase inhibition in the CA1 region of rat hippocampus on spatial localization was investigated in the Morris water maze.

Materials and methods

Rats cannulated in the CA1 region of their hippocampus received bilateral injections of vehicle (saline) or N-omega-nitro L-arginine methyl ester (L-NAME), a NO synthase inhibitor (50,100 and 200 microgram/0.5 microlitre) through the cannulae 30 minutes before training each day. Animals were subjected to 5 days of training in the Morris water maze; 4 days with the invisible platform to test spatial learning and the 5th day with the visible platform to test motivation and sensorimotor coordination.

Results

The results showed dose-dependent increases (p<0.001) in escape latency, traveled distance, heading angle, and dose-dependent decreases (p<0.01) in target quadrant entries in L-NAME-received groups as compared to the control group. This impairment was reversed by co-administration of mole equivalent doses of L-arginine, the NO precursor.

Conclusions

On the basis of the present data, it is concluded that processes mediated by NO synthesis in the hippocampus are essentially involved in spatial learning.

Authors’ Affiliations

(1)
Department of Physiology & Pharmacology, Pasteur Institute of Iran, Tehran, Iran

References

  1. Bon CL, Garthwaite J: On the role of nitric oxide in hippocampal long-term potentiation. J Neurosci. 2003, 23: 1941-1948.PubMedGoogle Scholar
  2. Martin SJ, Clark RE: The rodent hippocampus and spatial memory: from synapses to systems. Cell Mol Life Sci. 2007, 64: 401-431. 10.1007/s00018-007-6336-3.View ArticlePubMedGoogle Scholar
  3. Fin C, da Cunha C, Bromberg E, Schmitz PK, Bianchin M, Medina JH, Izquierdo I: Experiments suggesting a role for nitric oxide in the hippocampus in memory processes. Neurobiol Learn Mem. 1995, 63: 113-115. 10.1006/nlme.1995.1011.View ArticlePubMedGoogle Scholar
  4. Blokland A, de Vente J, Prickaerts J, Honig W, Markerink-van Ittersun M, Steinbusch H: Local inhibition of hippocampal nitric oxide synthase does not impair place learning in the Morris water escape task in rats. Eur J Neurosci. 1999, 11: 223-232. 10.1046/j.1460-9568.1999.00431.x.View ArticlePubMedGoogle Scholar

Copyright

Advertisement