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Post-training nitric oxide synthase inhibition in the CA1 region of rat hippocampus does not impair spatial memory consolidation
© Azizi et al.; licensee BioMed Central Ltd. 2008
Published: 17 April 2008
The highest levels of Nitric oxide (NO) throughout the body are found in neurons, where NO functions as a neuromodulator and appears to be involved in a variety of different neuronal functions, including learning and memory . A number of behavioral studies have provided evidence for the involvement of NO in the early stages of memory processing [2, 3]. Considering the critical role of the hippocampus in the processing of spatial information , the present study was designed to determine if post-training NO synthase inhibition in the CA1 region of rat hippocampus could affect spatial memory consolidation.
Materials and methods
Adult male rats were implanted with bilateral intra CA1 guide cannulae and were trained in the standard hidden platform version of the Morris water maze and given immediate post-training infusions of vehicle (saline) or N-omega-nitro L-arginine methyl ester (L-NAME), an NO synthase inhibitor (50,100 and 200 microgram / 0.5 micro liter) through the cannulae. A probe trial was done 24 hours later to test memory for the platform location.
All rats learned to find the platform on Day 1. But, there was no significant difference in spent time and traveled distance near the trained platform location and quadrant entries during the probe trial among the groups.
This work demonstrates that local NO synthase inhibition failed to affect performance in the rats that had previously acquired the task. Therefore it seems that NO synthesis in the CA1 hippocampal region is not crucial in consolidation of spatial memory in the Morris water maze learning task.
- Bredt DS: Endogenous nitric oxide synthesis: biological functions and pathophysiology. Free Radic Res. 1999, 31: 577-596. 10.1080/10715769900301161.View ArticleGoogle Scholar
- Martin SJ, Clark RE: The rodent hippocampus and spatial memory: from synapses to systems. Cell Mol Life Sci. 2007, 64: 401-431. 10.1007/s00018-007-6336-3.View ArticleGoogle Scholar
- Medina JH, Izquierdo I: Retrograde messengers, long-term potentiation and memory. Brain Res Brain Res Rev. 1995, 21: 185-194. 10.1016/0165-0173(95)00013-5.View ArticleGoogle Scholar
- Noda Y, Yamada K, Nabeshima T: Role of nitric oxide in the effect of aging on spatial memory in rats. Behav Brain Res. 1997, 83: 153-158. 10.1016/S0166-4328(97)86060-3.View ArticleGoogle Scholar
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