Skip to main content


  • Poster presentation
  • Open Access

The effects of histaminergic system in nucleus accumbens of rats in the plus-maze test of anxiety-like behaviours

  • 1,
  • 1 and
  • 2
Annals of General Psychiatry20087 (Suppl 1) :S225

  • Published:


  • Histamine
  • Ranitidine
  • Nucleus Accumbens
  • Elevated Plus Maze
  • Anxiolytic Effect


There are several studies indicating that histamine may elicit modulatory influence on anxiety-related behaviours both in animals and humans [14]. The activity of histaminergic pathways subserving corticolimbic structures is enhanced in response to fear-evoking and other stressful stimuli. The nucleus accumbens (NAc) belongs to the mesolimbic system and is a major component of the ventral striatum of rat. The elevated plus maze (EPM) was used to assess anxiolytic behaviour.

Materials and methods

In the present study, the anxiolytic effect of bilateral injections of the histamine into the NAc was examined in the EPM. histamin (0.01, 0.1, 1 microgram/rat), pyrilamine and ranitidine (0.001, 0.01, 0.1, 1 microgram/rat) were injected i.c.v. at the doses. Animals used in these experiments were male Wistar rats weighing 220-280 g at the time of surgery. Eight animals were used in each experiment. Animals were bilaterally cannulated in the NAc by stereotaxic instrument and were allowed to recover 1-week before behavioral testing.


Bilateral intra-NAc injections of the different doses of histamine, pyrilamine and ranitidine increased the percentage of open arm time (%OAT) and open arm entries (%OAE). The data may indicate that histamine produced a significant anxiolytic effect without the significant changes in the locomotor activity.


In conclusion, the NAc may be involved in histamine, pyrilamine and ranitidine-induced anxiolytic behavior. Generally we can conclude that effects of histaminergic system of NAc anxiolytic is like behavior of neuromedolatory and via the effect on other neurotransmitors releasing.



The authors are thankful to the Vice Chancellor of Research, Tehran University of Medical Sciences for financial support.

Authors’ Affiliations

Department of Animal Biology, Science and Research Campus, Islamic Azad University, Poonak, Tehran, Iran
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


  1. Zarrindast MR, Torabi M, Rostami P, Fazli-Tabaei S: The effects of histaminergic agents in the dorsal hippocampus of rats in the elevated plus-maze test of anxiety. Pharmacol Biochem Behav. 2006, Epub, 85 (3): 500-6. 10.1016/j.pbb.2006.09.019. Nov Epub 2006 Nov 15Google Scholar
  2. Rostami P, Hajizadeh-Moghaddam A, Zarrindast MR: The effects of histaminergic agents in the ventral hippocampus of rats in the plus-maze test of anxiety-like behaviours. Physiol Behav. 2006, Epub, 87 (5): 891-6. 10.1016/j.physbeh.2006.02.001. May 30 Epub 2006 Mar 6Google Scholar
  3. Zarrindast MR, Moghadam AH, Rostami P, Roohbakhsh A: The effects of histaminergic agents in the central amygdala of rats in the elevated plus-maze test of anxiety. Behav Pharmacol. 2005, 16 (8): 643-9. 10.1097/00008877-200512000-00007. DecView ArticlePubMedGoogle Scholar
  4. Zarrindast MR, Rostami P, Zarei M, Roohbakhsh A: Intracerebroventricular effects of histaminergic agents on morphine-induced anxiolysis in the elevated plus-maze in rats. Basic Clin Pharmacol Toxicol. 2005, 97 (5): 276-81. 10.1111/j.1742-7843.2005.pto_116.x. NovView ArticlePubMedGoogle Scholar


© Shalmani et al.; licensee BioMed Central Ltd. 2008

This article is published under license to BioMed Central Ltd.